- Biopolymers Services
- Proteomics Sample Prep and LC-MS
- TMT Sample Prep and LC-MS
- LC-MS only, Data Analysis and Other Services
Biopolymers Services
| Service | KI | MIT (non-KI) |
|---|---|---|
| Peptide Synthesis | ||
| 5 umol synthesis, per amino acid | $9.00 | $13.75 |
| 0.1 mmol synthesis, per cycle | $34.00 | $52.00 |
| 0.1 mmol synthesis set up | $86.00 | $131.50 |
| 0.3 mmol synthesis, per cycle | $49.50 | $75.75 |
| 0.3 mmol synthesis set up | $172.00 | $263.00 |
| 0.5 mmol synthesis, per cycle | $86.00 | $131.50 |
| 0.5 mmol synthesis set up | $255.00 | $390.00 |
| Multipep modification labor, per hour | $64.00 | $98.00 |
| Manual modification labor, per hour | $143.00 | $219.00 |
| SPOT synthesis | $1290.00 | $1974.00 |
| Di-sulfide cyclization (Type 1) | $322.00 | $492.75 |
| Di-sulfide cyclization (Type 2) | $97.00 | $148.50 |
| MALDI | ||
| MS run | $43.00 | $65.75 |
| Sample desalting | $43.00 | $65.75 |
| Additional mode run | $43.00 | $65.75 |
| HPLC | ||
| Analytical run | $36.00 | $55.00 |
| Purification up to 10 mg | $187.00 | $286.00 |
| Purification up to 100 mg | $373.00 | $570.75 |
| Desalting dye-labeled oligo | $43.00 | $65.75 |
Proteomics Sample Prep and LC-MS
| Service | KI | Non-KI (MIT) | Additional Sample (KI) |
Additional Sample (Non-KI) |
|---|---|---|---|---|
| Proteomics Sample Preparation | ||||
| Simple digest (gelbands; pure proteins) | $82.00 | $164.00 | $24.00 | $48.00 |
| Medium complex digest (gellanes; IPs); normally includes a C18 cleanup | $115.00 | $230.00 | $35.00 | $70.00 |
| Complex digest (lysates); includes cleanup* | $173.00 | $346.00 | $52.00 | $104.00 |
| Phosphorylation (cleanup and enrichment) | $116.00 | $232.00 | $58.00 | $116.00 |
| Tyrosine phosphorylation | $173.00 | $346.00 | $87.00 | $174.00 |
| Other PTM enrichment | $348.00 | $696.00 | $173.00 | $346.00 |
| Additional cleanup (C18 cleanup), per sample | $18.00 | $36.00 | N/A | N/A |
| Protein concentration measurements | $23.00 | $46.00 | $12.00 | $24.00 |
| Combined Prices (Sample Preparation/LC-MS/Data Analysis) | ||||
| Simple digest | $254.00 | $508.00 | 83.00 | $166.00 |
| Medium complex digest | $318.00 | $636.00 | $122.00 | $244.00 |
| Complex digest | $405.00 | $810.00 | $168.00 | $336.00 |
| Simple digest phosphorylation | $486.00 | $972.00 | $140.00 | $280.00 |
| Medium complex digest phosphorylation | $550.00 | $1100.00 | $180.00 | $360.00 |
| Complex digest phosphorylation | $636.00 | $1272.00 | $226.00 | $452.00 |
| Complex digest tyrosine phosphorylation | $695.00 | $1390.00 | $254.00 | $508.00 |
| Complex digest PTM | $868.00 | $1736.00 | $342.00 | $684.00 |
*Normally includes using a STrap, precipitation, or other C18 based method, more enzyme, could include protein conc measurement.
TMT Sample Prep and LC-MS
| Service | KI | Non-KI (MIT) |
|---|---|---|
| TMT Sample Preparation | ||
| TMT6 (100ug) | $579.00 | $1,158.00 |
| TMT10 (100ug) | $1,062.00 | $2,124.00 |
| TMT16 (100ug) | $1,544.00 | $3,088.00 |
| TMT6 (400ug) | $1,013.00 | $2,026.00 |
| TMT10 (400ug) | $1,857.00 | $3,714.00 |
| TMT16 (400ug) | $2,702.00 | $5,404.00 |
| TMT Experiments Calculated with 100ug Sample Sizes | ||
| TMT6 (IP) | $1,187.00 | $2,374.00 |
| TMT10 (IP) | $1,808.00 | $3,616.00 |
| TMT6 (lysate) | $1,361.00 | $2,722.00 |
| TMT10 (lysate) | $2,052.00 | $4,104.00 |
| TMT16 (lysate) | $2,847.00 | $5,694.00 |
| TMT6 (phospho experiment) | $1,880.00 | $3,760.00 |
| TMT10 (phospho experiment) | $2,804.00 | $5,608.00 |
| TMT16 (phospho experiment) | $3,946.00 | $7,892.00 |
| TMT6 (tyrosine experiment) | $2,084.00 | $4,168.00 |
| TMT10 (tyrosine experiment) | $3,123.00 | $6,246.00 |
| TMT16 (tyrosine experiment) | $4,438.00 | $8,876.00 |
| TMT6 (other PTM experiment) | $2,691.00 | $5,382.00 |
| TMT10 (other PTM experiment) | $4,077.00 | $8,154.00 |
| TMT16 (other PTM experiment) | $5,914.00 | $11,828.00 |
LC-MS only, Data Analysis and Other Services
| Service | KI | Non-KI (MIT) |
|---|---|---|
| Mass Spectrometry | ||
| Simple sample (single proteins; single gel bands) | $58.00 | $116.00 |
| Medium complex sample (gel lanes; IPs) | $87.00 | $174.00 |
| Complex sample (lysate) | $116.00 | $232.00 |
| PRM analysis (for up to 20 peptides) | $58.00 | $116.00 |
| Data Analysis | ||
| ID search | $116.00 | $232.00 |
| Quantitation analysis; TMT; PRM; LFQ; SILAC | $116.00 | $232.00 |
| PTM analysis; for single protein manual verification | $116.00 | $232.00 |
| Small molecules, pK | $116.00 | $232.00 |
| MW Protein Analysis | ||
| MW protein analysis (LC/MS: QE analysis) | $92.00 | $184.00 |
| HPLC | ||
| HPLC fractionation (setup) | $231.00 | $462.00 |
| Small Molecule Analysis | ||
| Initial setup for Quantitation (PRM) on QE (high flow) small molecules | $231.00 | $462.00 |
| Calibration curve | $116.00 | $232.00 |
| MRM runs for PK work (per sample); after initial setup and calibration curve | $23.00 | $46.00 |
The above fee schedule is effective as of August 1, 2024.
Note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Services are billed at the rate listed on the date of service delivery.