Specifications
- Inverted Olympus X71 microscope with XYZ nano-motion stage with 18 mm of XY travel
- Environmental chamber with temperature control and CO2 enrichment
- Solid state LED illumination (SSI) module coupled to a fiber optic cable
- 405 nm,488 nm, 512 nm, and 568 nm lasers for TIRF and Photokinetics
- LED transmitted light source
- Objectives: 10X, 20X, 40X, 60X, 100X, 100X TIRF
- Softworx software for image acquisition and deconvolution
- Sensitive back-thinned sCMOS camera
Fluorescence filter sets
Excitation-Emission | Probes & Fluorochromes | Sample |
---|---|---|
390/435 | DAPI, Hoechst, Coumarin | Fixed cells |
475/528 | FITC, Alexa488, GFP | Fixed/live cells |
542/585 | Alexa546, Alexa 555, Rhodamine, Texas Red, Phycoerythrin | Fixed/live cells |
632/685 | Cy5, Alexa 647 | Fixed/live cells |
438/465 | CFP | Live cells |
470/525 | EGFP | Live cells |
513/559 | YFP | Live cells |
575/630 | Alexa595, mCherry | Fixed/live cells |